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KMID : 0604520170430020175
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Journal of the Society of Cosmetic Scientists of Korea
2017 Volume.43 No. 2 p.175 ~ p.187
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Antioxidant Effect and Component Analysis of Cardiospermum halicacabum Leaf Extracts
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Jeong Hyo-Jin
Kim A-Rang
Lee Keon-Soo
Park So-Hyun
Shin Hyuk-Soo
Lee Sang-Rae
Song Ba-Reum
Lee Yun-Ju
An Hyun-Jin
Lee Jae-Duk
Park Soo-Nam
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Abstract
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In this study, the antioxidant effect and component analysis for extract and fractions of Cardiospermum halicacabum leaf were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried C. halicacabum leaf. The yields of extract and fractions were 16.4, 0.9 and 0.3% per dried powder, respectively. DPPH (1,1-phenyl-2-picrylhydrazyl) radical scavenging activity (FSC50) of ethyl acetate fraction (92.5 μg/mL) was the greatest radical scavenging activity, but lower than (+)-α-tocopherol (8.9 μg/mL). In reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, OSC50) on ROS generated in Fe3+-EDTA/H2O2 system, aglycone fraction (4.2 μg/mL) was the highest total antioxidant capacity and similar to L-ascorbic acid (1.5 μg/mL). The cellular protective effects of C. halicacabum leaf extract and fractions on the 1O2-induced cellular damage of human erythrocytes were exhibited at all concentration-dependent (5.0-25.0 μg/mL). Especially, aglycone fraction (τ50, 76.4 min) in 25.0 μg/mL showed the most protective effect among extracts. Components of the ethyl acetate fraction obtained from C. halicacabum extracts were analyzed by TLC, HPLC chromatogram and LC/ESI-MS. Results showed that the ethyl acetate fraction contained some flavonoids, such as apigenin-7-O-glucuronide, apigenin-7-glucosdie and quercitrin hydrate. These results suggest that the extracts and fractions of C. halicacabum leaf may be applied as antioxidant functional cosmetic raw materials.
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KEYWORD
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Cardiospermum halicacabum , antioxidant , reactive oxygen species , component analysis
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